Effect of Information Channels on Covid-19 Vaccine Acceptance among University Students of Rawalpindi/Islamabad: A Cross Sectional Study

ABSRACT Objectives: To find the effect of information channels on Covid-19 acceptance among university students of Rawalpindi/ Islamabad. Study Design: Cross sectional analytic study. Place and Duration of Study: Study was conducted in four imminent Universities in Rawalpindi/Islamabad, Air University, Rawalpindi Medical University, Riphah University and Quaid-e-Azam University, Rawalpindi, from May to Aug 2022. Methodology: Participants from four universities in Rawalpindi/Islamabad Pakistan, were recruited for this current research from May to August 2022. A sample size of 377±14 was computed using the Rao soft sample size calculator, maintaining a 5% margin of error and a 95% confidence interval a population proportion (50%). Non-probability convenient sampling was used. Data was analysed using SPSS version 26. Results: There were 377 students recruited, out of which majority 207(55%) were female. Most of the students were in the Medicine and allied fields 227(60.2%). Almost, half of the respondents (44.56%) received information about the COVID-19 vaccine from mobile networks. The level of trust in vaccine information was also highest for Mobile Networks (31.30%). Participants' perceptions of the covid 19 risk, 156(41%) revealed high concerns for infecting family and friends with the virus. There was a significant association of female gender (p<0.05) and being from the Medicine and allied fields (p=p<0.05) with vaccine acceptance. Conclusion: In our study the mobile and traditional media were reported as the main sources of information about the COVID-19 vaccination however they were not significantly associated with vaccine acceptance. Female gender, education level and being from the Medicine and allied fields was associated with intention to be vaccinated.

Detection of SARS-CoV-2 using real-time polymerase chain reaction in different clinical specimens: A critical review.

Coronavirus disease 2019 (COVID-19) is a disease caused by a new strain of coronavirus named as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Globally, since the outbreak, more than seven million confirmed cases of COVID-19 have been reported. The rapid spread and increase in the number of new cases is due to person-to-person transmission. To further control its transmission, early laboratory diagnosis of both asymptomatic and symptomatic patients is crucial. Presently, the COVID-19 diagnosis of infected individuals is dependent on computed tomography scanning and real-time polymerase chain reaction (PCR). The latter is considered more sensitive and efficient for early diagnosis. In this review, general comparisons are made (cases, fatality rate, incubation period, clinical features, and reservoirs) and diagnostic laboratory procedures (specimens, extraction methods, and positive rates by real-time PCR) are compared between SARS, Middle East Respiratory Syndrome, and SARS-2. In total, 8982 SARS-2 suspected patients specimen data were retrieved, in which 40.9% (n = 3678) were detected as positive by real-time PCR. The specimen-wise high detection rate was observed from bronchoalveolar lavage, followed by saliva, nasal swabs, and sputum. As the COVID-19 cases are persistently increasing, the selection of appropriate specimens and laboratory assay would help in rapid and timely diagnosis.

RECURRENT COVID-19 INFECTION IN RECOVERED PATIENTS (A CRITICAL REVIEW)

The pandemic of Coronavirus Disease 2019 (COVID-19) is still grooming throughout the world. However, the number of recovered patients with COVID-19 is also increasing day by day. Some discharged patients from hospitals had shown fever and radiological abnormalities again along with positive real-time reverse transcriptase-polymerase chain reaction (RT-PCR). This might be due to relapse of COVID-19 in recovered patients, secondary bacterial infection, the false-positive result after discharging, or a false negative at discharging yet to determine. In this review, a total of seven studies retrieved, in which 1052 COVID-19 infected patients were studied and followed them for one to six weeks. Among 1,052 patients, 12.1% (n=127) patients were found re-infected having positive RT-PCR with different clinical samples including nasopharyngeal swab, an anal swab, throat swab, and sputum samples. All re-infected patients were found with mild to moderate clinical symptoms. These findings suggest that recurrence of COVID-19 is exist. It is important to develop diagnostic tools further to avoid the false negative or positive results. Moreover, followup studies are required to determine the reason behind the recurrence of COVID-19 in recovered. Besides, we suggest because of the current review, further management of discharged patients is crucial especially for immunocompromised patients, old age patients, or patients with comorbidities. The discharge criteria should be ensured for a recovered patient to prevent the relapse of COVID-19.

ROLE OF IMAGING TECHNOLOGY AND CURRENT STATUS OF CORONA VIRUS DISEASE 2019 (COVID-19): A CRITICAL REVIEW

Lately, there was an outbreak of Coronavirus disease (COVID-19) in Wuhan, a city of province Hubei, China. The infectious and transferrable virus originated in bats, seafood and animals. COVID-19 belongs to Coronviradae family. Severe Acute Respiratory Syndrome (SARS) and Middle East Respiratory Syndrome (MERS) also belong to this family and their outbreak occurred in China and Saudi Arabia back in 2003 and 2012. number of deaths due to COVID-19 is much greater than that of SARS and MERS. The he clinical symptoms of infected individuals from virus are almost the same but diagnostically they are different from one another. Reverse transcription polymerase chain reaction (RT-PCR) laboratory technique is a standard test for evaluation and confirmation of COVID-19 disease. In early stages, imaging modalities can be used for screening purposes. X-Ray is used to identify the gross changes within the chest region while Computed Tomography Scanning (CT Scan) chest is used for detailed modification which occurs due to coronavirus. CT scan evaluates the different characteristics in the individuals infected from COVID-19 such as presence of bilateral ground glass opacities, presence of diffuse consolidation, presence of nodules with halo sign, enlarge thoracic lymph nodes, presence of thicken inter-lobar septa, presence of bronchiectasis and pleural effusion. Each lung lobe was assigned different score on the basis of lobe area involvement. A lobe having no involvement is of score 0, minimal involvement lobe score is 1, mild involvement lobe score is 2, moderate involvement lobe score is 3, and severe involvement lobe score is 4. The lobes are assessed for different degree of involvement and then are divided as none (0%), minimal (1% to 25%), mild (26% to 50%), moderate (51% to 75%) and severe 76% to above level. These all techniques are very effective and responsible for the exact location of the area involved and also show the extension of pathology that can only be possible on CT scan. CT scan not only evaluates the disease but it also provides the assessment of the disease severity.